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KMID : 1234420080360030221
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 3 p.221 ~ p.226
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Expression of Human Heavy-Chain and Light-Chain Ferritins in Saccharomyces cerevisiae for Functional Foods and Feeds
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Han Hye-Song
Lee Jung-Lim
Park Si-Hong
Kim Jae-Hwan
Kim Hea-Yeong
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Abstract
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To produce human ferritins in yeast, human H-chain and L-chain ferritins were amplified from previously cloned vectors. Each amplified ferritin gene was inserted into the pYES2.1/V5-His-TOPO yeast expression vector under the control of the GAL1 promoter. Western blot analysis of the recombinant yeast cells revealed that H-and L-chain subunits of human ferritin were expressed in Saccharomyces cerevisiae. Atomic absorption spectrometry (AAS) analysis demonstrated that the intracellular content of iron in the ferritin transformant was 1.6 to 1.8-fold higher than that of the control strain. Ferritin transformants could potentially supply iron-fortified nutrients for food and feed.
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KEYWORD
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Atomic absorption spectrometry, human ferritin, iron, Saccharomyces cerevisiae
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